Functional morphological alterations of human blood platelets induced by oxidized low density lipoprotein

The effect of oxidized low density lipoprotein (LDL) on the functional morphology of human platelets in vitro was studied by means of transmission electron microscopy. The washed platelets, stimulated by oxidized LDL (50-300 micrograms protein/ml), showed disc-sphere transformation, centralization of granules and complete degranulation in a dose- and time-dependent manner. A cytodamage in platelet membrane was induced by oxidized LDL leading to a lower electron density of cytoplasm compared to control. The morphological observations were supported by an analysis of the platelet shape-change parameter. Since the shape change, induced by oxidized LDL (50 micrograms/ml), was inhibited by a preincubation of platelets with staurosporine (10 nM), the protein kinase C was probably involved in the platelet activation initiated by oxidized LDL. The present results suggest that oxidized LDL could contribute to pathological thrombosis and atherogenesis by activating platelets.