[Mossbauer data on the effect of NAD.H on the state of iron in bacteria]

The state of Fe of bacterial cultures of different systematic positions (Bacillus megaterium, Bacillus polymyxa, Pseudomonas putida, Pseudomonas fluorescens, Alcaligenes faecalis, Arthrobacter siderocapsulatus) grown on the medium containing Fe(III) citrate (up to 100 mg/l) with additional or without NAD.H was studied. The samples were in damp air-dry, second moistened, dried at 383 K states. Spectra have been obtained at 290 K and 100-200 K. The studied microorganisms have two types of atoms of Fe(III) which dissociated into protoplasm or into cell slug at damp state. All studied microorganisms except Arthrobacter siderocapsulatus do not reduce iron at all variants of experiments. Arthrobacter siderocapsulatus reduces about 50% of cell iron only the presence of NAD.H creating two types of Fe(II) complexes firmly connected with cell.