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Comparison of solution structure of free and complexed lac operator by molecular modelling with NMR constraints
E Gincel1, G Lancelot, J C Maurizot
1CBM-CNRS, Orléans, France.
Biochimie
|January 1, 1994
Abstract:
The structure difference between the free operator of the lac system d(GCTCACAAT).d(ATTGTGAGC) and the same operator complexed to the headpiece of the lac repressor has been investigated by 2-D-1H NMR spectroscopy in conjunction with molecular modelling in internal coordinates (JUMNA). The free and complexed operator adopt both a right-handed B helical conformation, but a more detailed analysis of the conformational parameters using the Curves program shows striking differences in the groove geometries, the rises, the twists and the total bending.