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Gas chromatography applied to the lactulose-mannitol intestinal permeability test
Journal of Chromatography. B, Biomedical Applications
|April 1, 1994
Summary
This study validates a rapid and precise gas chromatographic method for measuring intestinal permeability using mannitol and lactulose probes. The validated method accurately quantifies these probes, aiding in the assessment of gut barrier function in various conditions.
Area of Science:
- Gastroenterology
- Analytical Chemistry
- Biomedical Science
Background:
- Intestinal permeability alterations are linked to illnesses, trauma, and sepsis.
- Increased intestinal permeability allows harmful substances and bacteria to translocate.
Purpose of the Study:
- To validate a capillary gas chromatographic (GC) method for determining mannitol and lactulose levels.
- To establish a precise and rapid assay for assessing intestinal permeability.
Main Methods:
- Capillary gas chromatography was employed for the determination of mannitol and lactulose.
- Method validation included linearity, recovery, and precision (intra- and inter-assay C.V.s).
- Normal values were established in a cohort of 25 healthy subjects.
Main Results:
- The GC method demonstrated linearity up to 3 g/l for mannitol and 300 mg/l for lactulose.
- Recovery from spiked samples ranged from 92% to 110%.
- Intra-assay C.V.s were 2.7% (mannitol) and 6.8% (lactulose); inter-assay C.V.s were 8.9% (mannitol) and 9.3% (lactulose).
Conclusions:
- The validated GC method is rapid, precise, and suitable for measuring intestinal permeability probes.
- This assay can aid in diagnosing and monitoring conditions associated with altered gut barrier function.