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Alterations in mouse macrophage migration: a function of assay systems, lymphocyte activation product preparation,
Abstract:
Supernatants from mouse spleen cell and peritoneal cell cultures were tested for the presence of lymphocyte activation products. Supernatants from mouse spleen cell and peritoneal cell cultures incubated with brucella antigens contained a macrophage migration inhibition factor(s) and a macrophage spreading factor(s) only if the cells were harvested from Brucella-infected mice. After dialysis and freeze-drying, the supernatants were fractionated by preparative acrylamide-gel electrophoresis. Three fractions with lymphocyte activation product activity were obtained from the fractionated supernatants of mouse spleen cells and peritoneal cells harvested from Brucella-infected mice and cultured with brucella antigen. One fraction inhibited mouse macrophage migration from capillary tubes but not from agarose wells. A second fraction not only inhibited macrophage migration from both agarose wells and capillary tubes, but also contained an activity(s) that stimulated macrophage migration through Nuclepore filters and induced macrophage spreading. A third fraction timulated macrophage migration from agarose wells and also contained an activity(s) that stimulated macrophage migration through Nuclepore filters. Fractionated supernatants of mouse spleen cells and peritoneal cells harvested from uninfected mice incubated with and without brucella antigen, as well as of cells harvested from infected mice and not incubated with antigen, did not contain detectable lymphocyte activation products.
Insights
Researchers identified specific lymphocyte activation products in Brucella-infected mice. These factors influence macrophage migration and spreading, offering insights into cellular immune responses to bacterial infections.
Area of Science:
- Immunology
- Cell Biology
- Microbiology
Background:
- Cellular immune responses are crucial for combating bacterial infections.
- Lymphocyte activation products play a role in modulating immune cell functions.
- Brucella antigens are known to elicit specific immune responses.
Purpose of the Study:
- To identify and characterize lymphocyte activation products in response to Brucella antigens.
- To investigate the effects of these products on macrophage behavior.
- To understand the cellular mechanisms underlying immune responses in Brucella-infected mice.
Main Methods:
- Culture of mouse spleen and peritoneal cells with Brucella antigens.
- Fractionation of cell culture supernatants using preparative acrylamide-gel electrophoresis.
- Assays for macrophage migration inhibition, macrophage spreading, and migration through filters.
Main Results:
- Supernatants from infected mice, when cultured with antigen, contained macrophage migration inhibition and spreading factors.
- Three active fractions were isolated: one inhibited migration, a second inhibited migration and induced spreading/migration, and a third stimulated migration.
- No detectable lymphocyte activation products were found in cells from uninfected mice or in infected cells without antigen stimulation.
Conclusions:
- Specific lymphocyte activation products are generated in response to Brucella infection.
- These products differentially affect macrophage migration and spreading, indicating complex immune regulation.
- The findings contribute to understanding the cellular immunology of Brucella infections.