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A membrane filter technique for testing disinfectants

J Prince, C E Deverill, G A Ayliffe

    Journal of Clinical Pathology
    |January 1, 1975
    PubMed
    Summary
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    The membrane filter test effectively assesses phenolic and chloroxylenol disinfectants against Pseudomonas aeruginosa. This method allows for accurate counting of surviving organisms and easy removal of residual disinfectant.

    Area of Science:

    • Microbiology
    • Surface Disinfection
    • Antimicrobial Efficacy Testing

    Background:

    • Phenolic disinfectants and chloroxylenol are commonly used for surface disinfection.
    • Accurate assessment of disinfectant efficacy is crucial for infection control.
    • Standardized testing methods are needed to evaluate disinfectant activity.

    Purpose of the Study:

    • To evaluate the surface disinfecting activity of phenolic and chloroxylenol disinfectants.
    • To investigate the influence of organism type, inoculum size, and water hardness on disinfectant efficacy.
    • To assess the suitability of the membrane filter test for disinfectant evaluation.

    Main Methods:

    • A membrane filter technique was employed for disinfectant testing.
    • Pseudomonas aeruginosa was used as the test organism.

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  • Disinfectant exposure times were 2.5 and 8 minutes in 300 ppm hardness water.

    • Main Results:

    • The membrane filter test demonstrated effectiveness in assessing disinfectant activity.
    • Factors such as organism type, inoculum size, and water hardness were investigated.
    • All surviving organisms could be enumerated, and residual disinfectant was readily removed.

    Conclusions:

    • The membrane filter test is a valuable method for evaluating surface disinfectants.
    • This method offers advantages in quantifying surviving microorganisms and managing residual disinfectant.
    • The study provides insights into the efficacy of phenolic and chloroxylenol disinfectants.