Related Experiment Video
Updated: Aug 9, 2026

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
Published on: March 22, 2012
The third exoplasmic loop of the thyrotropin receptor is partially involved in signal transduction
Abstract:
We characterized the role of the three exoplasmic loops in the transmembrane region of the thyrotropin receptor (TSHR) by substituting each loop with the counterpart of beta 2-adrenergic receptor amino acid sequence. Mutant El3 with a substitution of the 3rd exoplasmic loop showed significant decrease in the maximal level and sensitivity of TSH-stimulated cAMP response despite good retention of TSH binding. These findings suggest that the third exoplasmic loop of the TSH receptor is partially involved in the signal transduction mechanism.
More Related Videos
11:25Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
Published on: October 4, 2017
08:27Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
Published on: January 7, 2019
Related Concept Videos
What are Second Messengers?
Insertion of Single-pass Transmembrane Proteins in the RER
Integral transmembrane proteins possess transmembrane and extra membrane domains. The transmembrane domains are primarily made of 20-25 hydrophobic amino acids arranged in a helical secondary confirmation. These...
Amplifying Signals via Second Messengers
Amplifying Signals via Enzymatic Cascade
Signal Transduction: Overview
Typically, signal transduction involves three...
Synthesis and Regulation of Thyroid Hormones
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The iodine is then...