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A T7 promoter vector with a transcriptional terminator for stringent expression of foreign genes
T Nishihara1, T Iwabuchi, T Nohno
1Department of Biochemistry, Kawasaki Medical School, Okayama, Japan.
Gene
|July 22, 1994
Abstract:
An expression vector, pKK233-2 [Amann and Brosius, Gene 40 (1985) 183-190], was modified by replacing the trc promoter with the T7 phi 10 promoter and introducing a strong transcriptional terminator upstream from this promoter. This modification successfully suppressed background transcription and resulted in strict dependence upon induction.