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Sequence of the gene encoding an alkaline serine protease of thermophilic Bacillus smithii
A Milano1, P L Manachini, C Parini
1Dipartimento di Genetica e Microbiologia, University of Pavia, Italy.
Gene
|July 22, 1994
Abstract:
The complete nucleotide sequence of the gene encoding an alkaline serine protease from Bacillus smithii has been determined. Degenerate oligodeoxyribonucleotide primers were used to prime the amplification of a 507-bp sequence of the gene. This sequence was successively used for constructing new primers applied in inverse polymerase chain reaction, using as template self-ligated DNA fragments. The deduced amino-acid sequence is compared to serine proteases from B. amyloliquefaciens, B. licheniformis, B. subtilis and Thermus aquaticus.