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Characterization of IS1201, an insertion sequence isolated from Lactobacillus helveticus
P Tailliez1, S D Ehrlich, M C Chopin
1Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
Gene
|July 22, 1994
Summary
IS1201, a novel insertion sequence from Lactobacillus helveticus, was identified and characterized. Its unique nucleotide sequence confirms its utility as a specific DNA probe for identifying Lactobacillus helveticus strains.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Insertion sequences (IS) are mobile genetic elements found in bacteria.
- Lactobacillus helveticus is a lactic acid bacterium with industrial and probiotic applications.
Purpose of the Study:
- To identify and characterize a novel insertion sequence, IS1201, from Lactobacillus helveticus.
- To evaluate the potential of IS1201 as a specific DNA probe for Lactobacillus helveticus identification.
Main Methods:
- Nucleotide sequencing of the insertion sequence IS1201.
- Analysis of open reading frame and protein homology.
- Determination of terminal inverted repeats and target site duplication.
- Estimation of copy number per genome.
Main Results:
- IS1201 is a 1387-bp insertion sequence encoding a transposase-like protein.
- It exhibits homology to transposases of related IS elements but has a unique nucleotide sequence.
- IS1201 possesses terminal inverted repeats and generates target site duplications.
- Copy number varies from 3 to 16 per Lactobacillus helveticus genome.
Conclusions:
- IS1201 is a distinct insertion sequence within the Lactobacillus helveticus genome.
- The unique sequence of IS1201 makes it a valuable and specific DNA probe for identifying Lactobacillus helveticus strains.