[Immunochemical studies on aldolase isoenzymes in human serum (author's transl)]

Analysis of human serum aldolase isoenzymes A, B and C activities was performed by means of a recently developed immunochemical assay. Isoenzyme patterns were established in 130 healthy subjects selected from a normal range research program. Total aldolase activity ranged from 0.8, 1.6, 2.5 U/L, Ald A from 0.6, 1.2, 1.9 U/L, Ald B 0.0, 0.2, 0.7 U/L, Ald C 0.0, 0.1, 0.4 U/L expressed as chi plus or minus 2 s range. Comparing the histograms of total aldolase activity in patients with acute hepatitis and normal controls, an almost identical frequency distribution in both groups was observed. However, histograms of aldolase isoenzyme B values of these groups were practically completely separated. Thus in contrast with total aldolase activity, the determination of aldolase isoenzyme B activity is a useful criterion in the diagnosis of acute hepatitis.