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Related Experiment Videos

Evaluation of pressure-driven captive bubble surfactometer

G Putz1, J Goerke, S Schürch

  • 1Cardiovascular Research Institute, University of California, San Francisco 94143.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|April 1, 1994
PubMed
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Modified captive bubble surfactometers accurately measure lung surfactant film properties, including surface adsorption rates and surface tension. This improved device simplifies construction and provides reliable data for surfactant research.

Area of Science:

  • Pulmonary physiology
  • Biophysical chemistry

Background:

  • Accurate measurement of lung surfactant properties is crucial for understanding respiratory function.
  • Existing methods for assessing surfactant film behavior can be complex or limited in scope.

Purpose of the Study:

  • To modify the captive bubble surfactometer for enhanced measurement of surface adsorption rates.
  • To simplify the construction of the captive bubble surfactometer.
  • To validate the modified device's accuracy against established methods.

Main Methods:

  • Modification of a standard captive bubble surfactometer.
  • Calibration using standards and dipalmitoylphosphatidylcholine monolayers.
  • Comparison of measurements with a Wilhelmy surface balance and cathetometer.

Related Experiment Videos

  • Assessment of rabbit lung surfactant properties.

    • Main Results:

    • Good agreement was found between the modified captive bubble surfactometer and standard measurement techniques.
    • The device accurately measured surface adsorption rates and film stability.
    • Rabbit lung surfactant exhibited rapid adsorption and low surface tension (< 5 mN/m) on second compression.

    Conclusions:

    • The modified captive bubble surfactometer is a reliable tool for assessing lung surfactant film properties.
    • The device facilitates accurate measurement of surface adsorption rates and dynamic surface tension.
    • This improved instrument aids in the study of pulmonary surfactant function.