Crystallization and preliminary X-ray diffraction studies of xylose isomerase from Thermoanaerobacterium...

Xylose isomerase from the thermophile Thermoanaerobacterium thermosulfurigenes strain 4B has been crystallized by vapour diffusion from Jeffamine ED 4000 as precipitant. The crystal symmetry is P2(1)2(1)2(1), with unit cell dimensions a = 85.6 A, b = 153.5 A and c = 158.5 A. The protein molecular mass and volume of the unit cell is consistent with the presence of a tetramer of the enzyme in the asymmetric unit. The crystals diffract X-rays from a synchrotron source to 1.7 A resolution.