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Related Experiment Videos

A protein complex required for signal-sequence-specific sorting and translocation

B Wiedmann1, H Sakai, T A Davis

  • 1Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.

Nature
|August 11, 1994
PubMed
Summary
This summary is machine-generated.

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The nascent-polypeptide-associated complex (NAC) prevents nascent polypeptides from incorrect interactions. NAC ensures proper protein targeting by regulating signal recognition particle (SRP) binding.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Protein Targeting

Background:

  • Nascent polypeptides emerging from ribosomes require regulation to prevent misinteractions.
  • The signal recognition particle (SRP) pathway targets proteins to the endoplasmic reticulum.
  • The role of cytosolic factors in SRP-mediated protein targeting is not fully understood.

Purpose of the Study:

  • To investigate the function of the nascent-polypeptide-associated complex (NAC) in protein targeting.
  • To determine how NAC influences the interaction between nascent polypeptides and the signal recognition particle (SRP).

Main Methods:

  • Purification of the nascent-polypeptide-associated complex (NAC).
  • In vitro assays using ribosomes, nascent polypeptides, and cytosolic extracts.

Related Experiment Videos

  • Analysis of signal recognition particle (SRP) crosslinking and protein translocation.

    • Main Results:

    • NAC binds to nascent polypeptide chains emerging from ribosomes, preventing premature interactions.
    • Depletion of NAC and other cytosolic factors allows SRP to bind nascent polypeptides lacking signal peptides.
    • Absence of NAC leads to inefficient mistranslocation of non-signal peptide proteins into the endoplasmic reticulum.
    • Reconstitution with NAC restores SRP specificity and accurate protein translocation.

    Conclusions:

    • NAC acts as a crucial regulator, ensuring that SRP only engages with polypeptides destined for translocation.
    • NAC maintains the fidelity of the secretory pathway by preventing off-target protein entry into the endoplasmic reticulum.