Related Experiment Video
Updated: Aug 16, 2026

Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes
Published on: January 7, 2013
Protein kinase C (PKC)-mediated protein phosphorylation in rat aortic smooth muscle cells is enhanced by allylamine
1Department of Physiology and Pharmacology, College of Veterinary Medicine, Texas A & M University, College Station 77843-4466.
Abstract:
The profile of endogenous protein phosphorylation mediated by protein kinase C (PKC) was examined in cell fractions prepared from subcultured aortic smooth muscle cells (SMCs) isolated from rats treated with 70 mg/kg allylamine (AAM) or tap water for 20 days. Increased phosphorylation of endogenous proteins was observed under unstimulated conditions in the particulate, but not cytosolic, fraction of cells from AAM-treated animals (i.e. AAM cells) relative to control cells. Although the same phosphorylation bands were observed in the particulate or cytosolic fraction of control and AAM cells following phorbol ester stimulation of the enzyme, enhanced PKC-mediated phosphorylation was observed in both fractions of AAM cells relative to control cells. Measurements of exogenous histone Type III-S phosphorylation by PKC following in vitro exposure of naive SMCs to 100 microM AAM for up to 60 min revealed that AAM selectively increased histone phosphorylation in the cytosolic fraction of SMCs. These results demonstrate that AAM treatment enhances PKC-mediated protein phosphorylation in rat aortic SMCs and raise the possibility that such alterations participate in the angiotoxic response to AAM.
Related Concept Videos
Protein Kinases and Phosphatases
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
PI3K/mTOR/AKT Signaling Pathway
GPCRs Regulate Adenylyl Cylase Activity
Two...
cAMP-dependent Protein Kinase Pathways
Calmodulin-dependent Signaling
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Nitric Oxide Signaling Pathway

