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The relationship between renin isoelectric forms and renin glycoforms
S A Katz1, J A Opsahl, P A Abraham
1Division of Nephrology, Hennepin County Medical Center, Minneapolis 55415.
The American Journal of Physiology
|July 1, 1994
Summary
Renin
Area of Science:
- Biochemistry
- Renal Physiology
Background:
- Active renin exists in multiple isoelectric and glycoforms.
- The relationship between these forms was previously unknown.
Purpose of the Study:
- To investigate the relationship between renin isoelectric forms and Concanavalin A (Con A) glycoforms.
- To determine if isoelectric form composition explains differential plasma clearance rates of renin glycoforms.
Main Methods:
- Shallow gradient isoelectric focusing to separate isoelectric forms.
- Concanavalin A (Con A) affinity chromatography to separate glycoforms.
- Compartmental modeling to predict plasma clearance rates.
Main Results:
- Each of the three rat kidney renin Con A glycoforms contained significantly different proportions of six isoelectric forms.
- Renin glycoforms with higher Con A affinity had proportionally fewer acidic isoelectric forms.
- Compartmental models accurately predicted differential plasma clearance rates based on isoelectric form proportions.
Conclusions:
- Renin glycoforms are composed of distinct proportions of isoelectric forms.
- Isoelectric form heterogeneity sufficiently explains differential renin glycoform plasma clearance.
- Renin's isoelectric and glycoform heterogeneity are closely related, likely due to variable mannose and sialic acid attachments.