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Isolation of prolylendopeptidase-inhibiting peptides from bovine brain
T Ohmori1, T Nakagami, H Tanaka
1Department of Health and Dietary Science, Nippon Meat Packers, Inc., Ibaraki, Japan.
Biochemical and Biophysical Research Communications
|July 29, 1994
Abstract:
The peptides inhibiting prolylendopeptidase were screened and isolated from bovine brain by monitoring the inhibition of prolylendopeptidase produced by Flavobacterium meningosepticum. The amino acid sequence of the peptide having the highest inhibitory activity was determined as Met-Pro-Pro-Pro-Leu-Pro-Ala-Arg-Val-Asp-Phe-Ser-Leu-Ala-Gly-Ala-Leu-Asn. This peptide showed the IC50 and Ki values of 38.4 and 8.6 microM, respectively, for prolylendopeptidase isolated from bovine brain.