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Physiologic modulation of bronchial epithelial cell barrier function by polycationic exposure

X Y Yu¹, B H Schofield, T Croxton

¹Department of Environmental Health Sciences, Johns Hopkins School of Hygiene and Public Health, Baltimore, Maryland 21205.

American Journal of Respiratory Cell and Molecular Biology

|August 1, 1994

Summary

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Cationic proteins, like poly-L-lysine, can disrupt bronchial epithelial barrier function by increasing solute flux and decreasing electrical resistance. This effect is reversible and may be regulated by anionic factors.

Area of Science:

Pulmonary Medicine
Cell Biology
Epithelial Physiology

Background:

Bronchial epithelial cells form a critical barrier in the lungs.
Inflammatory processes can release cationic proteins that compromise this barrier.

Purpose of the Study:

To investigate how cationic stimuli affect canine bronchial epithelial (CBE) cell barrier function.
To characterize the modulation of epithelial barrier integrity by polycations.

Main Methods:

CBE cells were cultured and assessed for differentiation.
Transepithelial mannitol flux and electrical resistance were measured.
The effects of poly-L-lysine (PLL) and heparin were evaluated.

Main Results:

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CBE cultures showed mucociliary differentiation.

Poly-L-lysine (PLL) increased mannitol flux and decreased electrical resistance in a dose-dependent manner.

These effects reversed spontaneously and were modulated by heparin.

Conclusions:

Cationic proteins can reversibly alter bronchial epithelial barrier integrity.
This modulation may involve interactions with anionic factors, suggesting a physiological regulatory mechanism.