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Nucleotide sequence encoding human pancreatic ribonuclease

M Seno1, J Futami, M Kosaka

  • 1Department of Bioengineering Science, Faculty of Engineering, Okayama University, Japan.

Biochimica Et Biophysica Acta
|August 2, 1994
PubMed
Summary
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Researchers isolated and sequenced complementary DNA (cDNA) for human pancreatic ribonuclease. This molecular biology study details the genetic blueprint for this important human enzyme.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Human pancreatic ribonuclease is an enzyme with potential therapeutic applications.
  • Understanding its genetic basis is crucial for further research and development.

Purpose of the Study:

  • To isolate and characterize the complementary DNA (cDNA) encoding human pancreatic ribonuclease.
  • To provide a detailed genetic sequence for the enzyme.

Main Methods:

  • Construction and screening of a human pancreas cDNA library.
  • DNA sequencing of the isolated cDNA clone.
  • Bioinformatic analysis to identify open reading frames and regulatory regions.

Main Results:

  • Isolation of a 1620 bp cDNA clone.

Related Experiment Videos

  • Identification of an open reading frame encoding a 128 amino acid mature protein.
  • Characterization of a 28 amino acid signal peptide sequence.
  • Identification of 5' and 3' untranslated regions.

    • Conclusions:

    • The complete cDNA sequence for human pancreatic ribonuclease has been determined.
    • This provides a foundational resource for protein expression and functional studies.
    • The identified genetic information facilitates further investigation into ribonuclease biology.