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tRNA regions which contact with the ribosomal poly(U)-programmed P-site
S A Nekhai1, D V Parfenov, E M Saminsky
1Division of Radiation and Molecular Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad region.
Biochimica Et Biophysica Acta
|August 2, 1994
Abstract:
Equilibrium binding affinity of yeast tRNA(Phe) for Escherichia coli poly(U)-programmed 70S ribosomal P-site was compared with corresponding affinities of several tRNA(Phe) 3'- and 5'-end-truncated derivatives, all containing the anticodon arm. Our findings strongly suggest that besides three 3'-terminal-CCA nucleotides (C74, C75 and A76), only the tRNA(Phe) anticodon arm (N28-N42) contains ribosomal P-site contact centers and that there are no such centers in the intermediate regions N1-N27 and N43-N73.