Studies on immobilization of aminoacylase on the functionalized polymethyl acrylate

Functionalized polymethyl acrylate was used for the immobilization of aminoacylase of Aspergillus oryzae. The effects of the degree of cross-linking, the amount of pore-forming agent and their mixing rates on the activity of immobilized aminoacylase were investigated. The results showed that the activity of the immobilized aminoacylase was the highest when the carrier was a combination of 20% cross-linking and 100% pore-forming agent. The effects of substrate concentration, ionic concentration, temperature and pH on the immobilized aminoacylase were compared with those on soluble aminoacylase, on the other hand, the effects of some organic solvents and protein denaturants on immobilized aminoacylase were also investigated. A column reactor packed with immobilized aminoacylase was used to resolve continuously N-acyl-DL-phenylalanine for a month, and 90% activity of immobilized aminoacylase was retained.