Related Experiment Videos
Isolation of a poplar and an Arabidopsis thaliana dihydrodipicolinate synthase cDNA clone
1Laboratorium voor Plantengenetica, Vrije Universiteit Brussel, St.-Genesius Rode, Belgium.
Plant Molecular Biology
|June 1, 1994
Abstract:
A poplar DHDPS cDNA clone has been isolated by functional rescue of the dapA-deficient AT997 mutant of Escherichia coli. By sequence comparison between the poplar and maize DHDPS cDNAs, two oligonucleotides were designed to perform polymerase chain reaction (PCR) on Arabidopsis thaliana genomic DNA. The PCR fragment was subsequently used to isolate an Arabidopsis DHDPS genomic and cDNA clone.