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[Measurement of the number of single-strand breaks in DNA of irradiated leukemia P-388 using alkaline denaturation]
Biulleten' Eksperimental'Noi Biologii I Meditsiny
|April 1, 1993
Abstract:
Methods, used for indirect determination of single-thread breaks' quantity in DNA, based on it's denaturation in alkali medium and further determination of double-thread DNA quantity by it's fluorescence has been adapted to the measuring of thread disturbances on leukemia P-388 cells. Influence of cells' and alkali concentrations and the time of denaturation of quantity of double-thread DNA has been investigated.