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Related Experiment Videos

A new bullous pemphigoid antigen

S Fujiwara1, K Kohno, A Iwamatsu

  • 1Department of Dermatology, Oita Medical University, Japan.

Dermatology (Basel, Switzerland)
|January 1, 1994
PubMed
Summary

Researchers identified a novel 450-kD antigen in subepidermal blistering disease. This antigen, expressed in keratinocytes, shows no homology to known human components, suggesting a new target for autoimmune blistering diseases.

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Area of Science:

  • Dermatology
  • Immunology
  • Molecular Biology

Background:

  • Subepidermal blistering diseases present clinical challenges, sometimes mimicking bullous pemphigoid.
  • Previous studies identified a patient's serum reacting to a specific 450-kD epidermal polypeptide.

Purpose of the Study:

  • To characterize the novel 450-kD antigen involved in subepidermal blistering disease.
  • To isolate and identify the cDNA encoding this antigen for further study.

Main Methods:

  • Immunoblot analysis to detect serum reactivity with epidermal antigens.
  • Immune screening of a cDNA expression library using patient serum.
  • Northern blot analysis to determine mRNA size and expression.

Main Results:

  • Patient serum exclusively reacted with a 450-kD epidermal polypeptide.
  • This antigen is present in human keratinocytes and squamous carcinoma cell lines.
  • A single cDNA clone encoding the antigen was isolated from a library of 2 million clones.
  • The corresponding mRNA is over 12 kb, and the deduced amino acid sequence shows no homology to known human proteins.

Conclusions:

  • A novel 450-kD epidermal antigen is implicated in a subepidermal blistering disease.
  • The isolation of its cDNA provides a basis for understanding its role in disease pathogenesis.
  • This antigen represents a potential new target for diagnostic and therapeutic strategies in autoimmune blistering conditions.

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