Related Experiment Video
Updated: Aug 15, 2026
Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
Published on: February 14, 2018
Differentiation of Cryptococcus neoformans serotypes A and D using creatinine dextrose bromothymol blue thymine
E A Irokanulo1, C O Akueshi, A A Makinde
1National Veterinary Research Institute, Vom, Nigeria.
Abstract:
A serotype differentiation of the encapsulated yeast Cryptococcus neoformans is described using creatinine dextrose bromothymol blue thymine (CDBT) medium. On CDBT medium C. neoformans serotype D grew as bright red colonies, turning the medium a bright orange after five days incubation at 28 degrees C. C. neoformans serotype A grew as pale colonies with no apparent colour effect on the medium. Serotypes B and C caused a slight greening of the medium. The reaction of the four serotypes of C. neoformans on CDBT medium is considered useful in the differentiation of the closely related serotype A and D.
