Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapid selection for serotonin-1A sensitivity in rats

D H Overstreet1, A H Rezvani, O Pucilowski

  • 1Skipper Bowles Center for Alcohol Studies, University of North Carolina School of Medicine, Chapel Hill 27599-7175.

Psychiatric Genetics
|January 1, 1994
PubMed
Summary

Researchers rapidly selected rat lines for distinct hypothermic responses to a serotonin agonist. This genetic selection for 5-HT1A sensitivity demonstrates rapid adaptation and suggests independent muscarinic receptor changes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "Paternal cannabis extract exposure in rats: Preconception timing effects on neurobehavioral effects in offspring" [Neurotoxicology 81 (2020) 180-188].

Neurotoxicology·2021
Same author

Long-term characterization of the Flinders Sensitive Line rodent model of human depression: Behavioral and PET evidence of a dysfunctional entorhinal cortex.

Behavioural brain research·2015
Same author

Monoamines and ovarian hormone-linked sexual and emotional changes: A review.

Archives of sexual behavior·2013
Same author

Gestational ethanol and nicotine exposure: effects on maternal behavior, oxytocin, and offspring ethanol intake in the rat.

Neurotoxicology and teratology·2008
Same author

Withdrawal emotional-regulation in infant rats from genetic animal models of depression.

Behavioural brain research·2008
Same author

Neonatal 6-hydroxydopamine lesions of the frontal cortex in rats: persisting effects on locomotor activity, learning and nicotine self-administration.

Neuroscience·2008

Area of Science:

  • Neuropharmacology
  • Behavioral Genetics
  • Animal Models

Background:

  • Serotonin-1A (5-HT1A) receptors play a crucial role in regulating body temperature.
  • Genetic variability in 5-HT1A receptor function can influence hypothermic responses.
  • Understanding these mechanisms is vital for developing targeted therapeutics.

Purpose of the Study:

  • To investigate the feasibility and speed of genetically selecting for differential hypothermic responses to a 5-HT1A agonist.
  • To characterize the selected rat lines' sensitivity to 8-hydroxy-2-(di-N-propylamino)tetralin (8-OH-DPAT).
  • To explore potential correlations between serotonergic and muscarinic receptor sensitivity.

Main Methods:

  • Utilized National Institutes of Health (NIH) heterogeneous stock (HS) rats.

Related Experiment Videos

  • Established genetically selected lines (HI and LO) for high and low hypothermic responses to 8-OH-DPAT, alongside a randomly bred control (RA) line.
  • Administered 8-OH-DPAT and oxotremorine to assess hypothermic effects across selected generations (S1 and S3).

    • Main Results:

    • Rapid divergence in hypothermic responses to 8-OH-DPAT was observed as early as the S1 generation.
    • By S3, the LO line exhibited a 0.5°C reduction and the HI line a 0.7°C increase in hypothermia compared to the RA line (p < 0.01).
    • Selected lines showed differing responses to oxotremorine, but these differences did not evolve with continued selection.

    Conclusions:

    • Genetic selection for 5-HT1A agonist-induced hypothermia is highly effective and occurs rapidly.
    • The study establishes distinct rat lines with differing sensitivities to 8-OH-DPAT.
    • Changes in muscarinic receptor sensitivity do not appear to directly parallel the observed rapid changes in serotonergic sensitivity.