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Expression of multidrug resistance-associated protein (MRP) in anaplastic carcinoma of the thyroid
I Sugawara1, T Arai, T Yamashita
1Department of Pathology, Saitama Medical School, Japan.
Abstract:
Although the incidence is very low, the prognosis of anaplastic carcinoma of the thyroid is very poor regardless of the results of various therapeutic trials. We found that the mechanism of anti-cancer drug resistance in anaplastic carcinoma of the thyroid was not explicable only in terms of expression of the mdr1 and its gene product, P-glycoprotein. Therefore, expression of multidrug resistance-associated protein (MRP) mRNA was examined in 11 anaplastic thyroid carcinomas and eight anaplastic thyroid carcinoma cell lines. High MRP mRNA expression was recognized in 7/11 and 8/8, respectively. Our results may contribute to elucidation of the mechanism of anti-cancer drug resistance in this neoplasm.
Insights
Anaplastic thyroid carcinoma shows poor prognosis. Multidrug resistance-associated protein (MRP) mRNA expression is high in these tumors, suggesting its role in anti-cancer drug resistance.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Anaplastic thyroid carcinoma (ATC) has a very poor prognosis despite therapeutic efforts.
- The mechanisms of anti-cancer drug resistance in ATC are not fully understood.
- P-glycoprotein (mdr1) expression alone does not explain drug resistance in ATC.
Purpose of the Study:
- To investigate the role of multidrug resistance-associated protein (MRP) mRNA expression in ATC.
- To elucidate the mechanisms of anti-cancer drug resistance in anaplastic thyroid carcinoma.
Main Methods:
- Quantitative analysis of MRP mRNA expression in 11 ATC tissue samples.
- Analysis of MRP mRNA expression in 8 ATC cell lines.
Main Results:
- High MRP mRNA expression was detected in 7 out of 11 (63.6%) ATC tissue samples.
- All 8 (100%) ATC cell lines exhibited high MRP mRNA expression.
Conclusions:
- MRP mRNA expression is frequently elevated in anaplastic thyroid carcinoma.
- This finding suggests that MRP plays a significant role in the drug resistance observed in ATC.
- Further research into MRP inhibition could offer new therapeutic strategies for ATC.