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Related Experiment Videos

Myogenic specification in somites: induction by axial structures

N Buffinger1, F E Stockdale

  • 1Stanford University, School of Medicine, CA 94305-5306.

Development (Cambridge, England)
|June 1, 1994
PubMed
Summary
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Myogenic specification, the development of muscle fibers in chick embryos, begins at stage 11 and is induced by the notochord and neural tube, leading to diverse fiber types.

Area of Science:

  • Developmental Biology
  • Embryology
  • Muscle Development

Background:

  • The process of myogenic specification, or the development of muscle fibers, is crucial for embryonic development.
  • Understanding the timing and inductive signals for myogenesis is essential for developmental biology.

Purpose of the Study:

  • To investigate the temporal and spatial patterns of myogenic specification in early chick embryos.
  • To determine the role of axial structures, specifically the notochord and neural tube, in inducing myogenesis.
  • To examine the establishment of myofiber diversity during early development.

Main Methods:

  • Organotypic explant cultures of chick somites and segmental plates.
  • Immunohistochemical staining using monoclonal antibodies against myosin heavy chain (MyHC).

Related Experiment Videos

  • Recombination experiments with axial structures (notochord, neural tube) and somites/segmental plates.

    • Main Results:

    • Myogenic specification initiates at Hamburger and Hamilton stage 11, initially in rostral somites, with caudal somites specified later.
    • The notochord and neural tube can induce myogenesis in unspecified somites.
    • The neural tube, but not the notochord, induces myogenesis in segmental plate explants.
    • Myofiber diversity (slow and fast fibers) is established early, even in unspecified somites when induced by axial structures.

    Conclusions:

    • Myogenic specification is an inductive process mediated by the notochord and neural tube.
    • This induction leads to the formation of diverse muscle fiber types.
    • The timing of specification and the inductive capacity of axial structures are key to muscle development.