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Growth-arrested dependence of aldolase A L-type mRNA expression in rodent cell lines
A Lupo1, P Costanzo, M De Rosa
1Dipartimento Biochimica e Biotecnologie Mediche, Facoltà di Medicina, Università degli studi di Napoli Federico II.
Abstract:
Two ubiquitous (L- and F-type) and one muscle-specific (M-type) mRNA species are generated by the human aldolase A gene. Despite the high degree of sequence similarities in the promoter region between human and rodents, no L-type mRNA expression has yet been found in the latter. Here we demonstrate that L-type aldolase A mRNA is expressed during the differentiation of mouse myogenic C2.7 and rat oligodendrocyte precursor CEINGE C13 cells. The L-type mRNA expression is increased during differentiation and is associated with cell-growth arrest caused by nocodazole treatment or serum deprivation in C2.7 and CEINGE C13 cells, respectively. The L-type aldolase A mRNA is correctly processed at the L1-L2 junction.