Related Experiment Videos
6-Fluoropyridoxol: a novel probe of cellular pH using 19F NMR spectroscopy
V D Mehta1, P V Kulkarni, R P Mason
1Department of Radiology, University of Texas Southwestern Medical Center, Dallas 75235-9058.
FEBS Letters
|August 1, 1994
Abstract:
6-Fluoropyridoxol was evaluated as an intracellular pH indicator. This molecule exhibits exceptional sensitivity to changes in pH (approximately 10 ppm acid/base shift) and a pKa approximately 8.2 appropriate for physiological investigations. Using 19F NMR spectroscopy we determined both intra- and extracellular pH in whole blood and confirmed the measurements using traditional techniques: ion-electrodes and 31P NMR spectroscopy.