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Updated: Jul 28, 2026

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors
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Published on: June 7, 2016

Internalization of the rat AT1a and AT1b receptors: pharmacological and functional requirements

S Conchon1, C Monnot, B Teutsch

  • 1Institut National de la Santé et de la Recherche Médicale Unité 36 Collège de France, Paris.

FEBS Letters
|August 8, 1994
PubMed

The capacity of the angiotensin II (AngII) agonist [Sar1]AngII, the antagonist [Sar1-Ile8]AngII and the non-peptidic antagonist DuP753 to undergo receptor internalization were studied in Chinese hamster ovary cells expressing rat AngII type 1a or 1b receptors (AT1a or AT1b) or a mutant of AT1a (Asn74) unable to couple G-protein. In this expression system, the ligand-induced internalization of rat AT1a and AT1b are similar. Moreover, peptidic ligands, either the agonist or antagonist, induce a significant internalization of AT1 receptors, but the non-peptidic antagonist DuP753 is far less potent. Finally, the normal internalization of the mutant Asn74 demonstrates that receptor activation and G-protein coupling are not required for AT1a internalization.

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