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Updated: Oct 8, 2026

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
Published on: January 4, 2017
Conjugal transfer of the vancomycin resistance determinant vanB between enterococci involves the movement of large
1Unité des Agents Antibactériens, CNRS EPJ0058, Institut Pasteur, Paris, France.
Abstract:
Resistance to various levels of vancomycin and susceptibility to teicoplanin in enterococci (VanB phenotype) is mediated by the vanB gene cluster. VanB-type resistance was transferred by intra- and inter-specific conjugation between different strains of Enterococcus. Analysis of SfiI-digested genomic DNA by zero integrated-field electrophoresis followed by Southern hybridization revealed vanB-containing chromosomal insertions of approximately 90-250 kb in the transconjugants. Thus, transfer of VanB-type resistance is associated with the movement of large genetic elements from chromosome to chromosome.
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