Related Experiment Video
Updated: Aug 19, 2026

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
Published on: January 4, 2017
Identification and nucleotide sequence analysis of a transfer-related region in the streptococcal conjugative
A O Kiliç1, M N Vijayakumar, S F al-Khaldi
1Oklahoma State University, Stillwater 74078.
Abstract:
To obtain a functional map of Tn5252, a 47.5-kb streptococcal conjugative transposon, a series of defined deletion and insertion mutations were introduced within the transposon. Interruptions at several regions were found to affect the conjugal transposition functions of the element in filter-mating experiments. The nucleotide sequence of the left terminus of Tn5252 showed two open reading frames, ORF1 and ORF2, adjoining the att site. The organization of this region and the structure of the predicted integrase encoded by ORF1 were found to be similar to those of other site-specific recombination systems.
More Related Videos
11:50High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection
Published on: December 18, 2017
08:19Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
Published on: July 7, 2020
Related Concept Videos
Overview of Transposition and Recombination
DNA-only Transposons
The donor site from where the transposon is excised is either degraded or...
LTR Retrotransposons
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
Transposons
Conjugation
Mechanism of Conjugation