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Partial characterization of Streptococcus suis type 2 hemolysin
I Feder1, M M Chengappa, B Fenwick
1Department of Pathology and Microbiology, College of Veterinary Medicine, Kansas State University, Manhattan 66506.
Journal of Clinical Microbiology
|May 1, 1994
Summary
Streptococcus suis type 2 hemolysin production was investigated. Hemolytic activity varied with growth conditions, showing reduced activity with fetal calf serum and increased activity with bovine serum albumin.
Area of Science:
- Microbiology
- Bacterial Pathogenesis
Background:
- Streptococcus suis is an important zoonotic pathogen.
- Hemolysins are virulence factors in many bacteria.
- The hemolytic activity of S. suis type 2 requires further characterization.
Purpose of the Study:
- To evaluate hemolysin production in Streptococcus suis type 2.
- To investigate factors affecting S. suis type 2 hemolysin activity.
- To identify S. suis serotypes capable of hemolysin production.
Main Methods:
- Photometric assay for hemolytic activity.
- Cultivation of S. suis in Todd-Hewitt broth and RPMI 1640 medium.
- Testing effects of temperature, atmosphere, chemicals, and enzymes on hemolysis.
Main Results:
- Nine of 23 S. suis serotypes tested produced hemolysins.
- Optimal hemolysis occurred at 40°C in 6% CO2 or anaerobically at 37°C.
- Fetal calf serum inhibited hemolysis, while bovine serum albumin enhanced it.
- Trypsin and amylase reduced hemolytic activity.
Conclusions:
- S. suis type 2 hemolysin is a secreted, thermolabile molecule.
- Hemolysin activity is dependent on growth conditions.
- Hemolysin production varies among S. suis serotypes.