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Related Experiment Videos

Partial characterization of Streptococcus suis type 2 hemolysin

I Feder1, M M Chengappa, B Fenwick

  • 1Department of Pathology and Microbiology, College of Veterinary Medicine, Kansas State University, Manhattan 66506.

Journal of Clinical Microbiology
|May 1, 1994
PubMed
Summary
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Streptococcus suis type 2 hemolysin production was investigated. Hemolytic activity varied with growth conditions, showing reduced activity with fetal calf serum and increased activity with bovine serum albumin.

Area of Science:

  • Microbiology
  • Bacterial Pathogenesis

Background:

  • Streptococcus suis is an important zoonotic pathogen.
  • Hemolysins are virulence factors in many bacteria.
  • The hemolytic activity of S. suis type 2 requires further characterization.

Purpose of the Study:

  • To evaluate hemolysin production in Streptococcus suis type 2.
  • To investigate factors affecting S. suis type 2 hemolysin activity.
  • To identify S. suis serotypes capable of hemolysin production.

Main Methods:

  • Photometric assay for hemolytic activity.
  • Cultivation of S. suis in Todd-Hewitt broth and RPMI 1640 medium.
  • Testing effects of temperature, atmosphere, chemicals, and enzymes on hemolysis.

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Main Results:

  • Nine of 23 S. suis serotypes tested produced hemolysins.
  • Optimal hemolysis occurred at 40°C in 6% CO2 or anaerobically at 37°C.
  • Fetal calf serum inhibited hemolysis, while bovine serum albumin enhanced it.
  • Trypsin and amylase reduced hemolytic activity.

Conclusions:

  • S. suis type 2 hemolysin is a secreted, thermolabile molecule.
  • Hemolysin activity is dependent on growth conditions.
  • Hemolysin production varies among S. suis serotypes.