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Cell-to-cell binding induced by different lectins

U Rutishauser, L Sachs

The Journal of Cell Biology

|May 1, 1975

Summary

This summary is machine-generated.

Cell-to-cell binding mediated by lectins like concanavalin A (Con A) depends on receptor density and mobility. Differences in lectin-induced cell binding are explained by receptor interactions and cell type, suggesting a bridge-formation mechanism.

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Area of Science:

Cell Biology
Immunology
Biochemistry

Background:

Cell-to-cell binding is crucial for biological processes.
Lectins mediate cell adhesion by binding to specific carbohydrate structures on cell surfaces.
Understanding lectin-induced cell interactions provides insights into cellular communication and immune responses.

Purpose of the Study:

To analyze cell-to-cell binding induced by various lectins (Concanavalin A, wheat germ, soybean, wax bean).
To investigate the influence of cell fixation and enzymatic treatments on lectin-mediated cell adhesion.
To elucidate the mechanisms underlying lectin-induced cell binding and agglutination.

Main Methods:

Single cells were tested for binding to lectin-coated cells immobilized on nylon fibers.

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Cells included lymphoma, myeloid leukemia, and normal fibroblast types.

Treatments involved glutaraldehyde fixation, trypsinization, and neuraminidase digestion.

Main Results:

Cell-to-cell binding was inhibited by dual glutaraldehyde fixation but often enhanced by single-cell fixation.
Trypsin treatment enhanced binding for Con A and wheat germ lectin with fibroblasts.
Neuraminidase increased soybean agglutinin binding only when both cells were treated.
Con A-induced binding was irreversible by saccharide inhibitors, unlike soybean and wheat germ lectin-induced binding.

Conclusions:

Lectin-induced cell binding is regulated by receptor density, mobility, and clustering.
Variations in cell binding and agglutination across cell types and lectins can be attributed to these receptor dynamics.
Cell-to-cell binding mediated by lectins involves the formation of multiple cell-lectin-cell bridges through receptor alignment.