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Related Experiment Videos

Helicobacter pylori: a fickle germ

L Cellini1, N Allocati, E Di Campli

  • 1Istituto di Medicina Sperimentale, Facoltà di Medicina e Chirurgia, Università G. D'Annunzio, Chieti, Italy.

Microbiology and Immunology
|January 1, 1994
PubMed
Summary

Helicobacter pylori transitions to non-culturable coccoid forms within days, especially under stress. These coccoid forms exhibit distinct protein profiles and potential resistance, impacting bacterial detection and eradication strategies.

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Area of Science:

  • Microbiology
  • Bacterial Morphology
  • Cellular Viability

Background:

  • Helicobacter pylori exhibits significant morphologic plasticity.
  • Understanding the transition to coccoid forms is crucial for diagnosing and treating infections.

Purpose of the Study:

  • To investigate the morphologic transformation of Helicobacter pylori from bacillary to coccoid forms.
  • To assess the culturability and potential resistance of coccoid forms.
  • To analyze proteomic differences between bacillary and coccoid morphologies.

Main Methods:

  • Culturing H. pylori in Brucella broth with fetal calf serum.
  • Incubation under microaerophilic and aerobic conditions.
  • Exposure to subinhibitory concentrations of omeprazole and roxithromycin.

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  • Heat and sonication treatments to assess viability.
  • Proteomic analysis of different morphologic forms.

    • Main Results:

    • Coccoid forms emerged within five days, showing decreased CFU/ml.
    • Coccoid forms were non-culturable in vitro after two weeks.
    • Coccoid morphology appeared earlier under aerobic conditions and with antibiotics.
    • Heat and sonication treatments were necessary for recovery of coccoid forms, indicating potential resistance.
    • Significant differences in protein patterns were observed between bacillary and coccoid forms.

    Conclusions:

    • Helicobacter pylori readily transitions to a non-culturable coccoid state.
    • Environmental stressors and subinhibitory antibiotic concentrations accelerate this transformation.
    • Coccoid forms possess distinct proteomic profiles and may exhibit enhanced resistance, posing challenges for eradication.