Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Extrachromosomal elements in tobacco plastids

J M Staub1, P Maliga

  • 1Waksman Institute, Rutgers, State University of New Jersey, Piscataway 08855-0759.

Proceedings of the National Academy of Sciences of the United States of America
|August 2, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Is bromodeoxyuridine resistance a consequence of cytokinin habituation in Nicotiana tabacum?

Planta·2014
Same author

Isolation of somatic hybrids by cloning Nicotiana heterokaryons in nurse cultures.

Planta·2014
Same author

An altered chloroplast ribosomal protein in a streptomycin resistant tobacco mutant.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Chloroplast transfer in Nicotiana based on metabolic complementation between irradiated and iodoacetate treated protoplasts.

Planta·2013
Same author

Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum + Nicotiana knightiana: correlation of resistance to N. tabacum plastids.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Streptomycin resistance is inherited as a recessive Mendelian trait in a Nicotiana sylvestris line.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013

Researchers discovered a novel extrachromosomal DNA minicircle, NICE1, in tobacco plastids. This unexpected finding opens avenues for engineering plastid genes and recovering mutations using shuttle plasmids.

Area of Science:

  • Plant molecular biology
  • Genetics
  • Molecular genetics

Background:

  • Higher plant plastid genomes are typically circular, double-stranded DNA molecules present in multiple copies.
  • Extrachromosomal DNA elements are generally absent in higher plant plastids.

Purpose of the Study:

  • To characterize a novel extrachromosomal DNA minicircle (NICE1) found in tobacco plastids.
  • To investigate the potential of shuttle plasmids for engineering plastid genomes and recovering mutations.

Main Methods:

  • Transformation of tobacco (Nicotiana tabacum) plastids.
  • Construction of shuttle plasmids containing NICE1 sequences.
  • Particle bombardment for reintroduction of shuttle plasmids into plastids.
  • Analysis of homologous recombination between shuttle plasmids and the plastid genome.

Related Experiment Videos

  • Recovery and characterization of recombinant DNA in Escherichia coli and in progeny plants.

    • Main Results:

    • An 868-bp plastid DNA minicircle, NICE1, was unexpectedly formed via homologous recombination during tobacco plastid transformation.
    • Shuttle plasmids containing NICE1 sequences were maintained extrachromosomally in re-transformed plastids.
    • Homologous recombination occurred between shuttle plasmids and the main plastid genome.
    • Recombination products were successfully recovered and characterized.

    Conclusions:

    • Shuttle plasmids can be utilized to engineer plastid genes without integrating foreign DNA.
    • This system allows for the recovery of plastid mutations in Escherichia coli.
    • The discovery of NICE1 provides a new tool for plastid genetic manipulation.