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Related Experiment Videos

Turkey gizzard caldesmon molecular weight and shape

W F Stafford1, J M Chalovich, P Graceffa

  • 1Boston Biomedical Research Institute, Muscle Research Group, Massachusetts 02114-2500.

Archives of Biochemistry and Biophysics
|August 15, 1994
PubMed
Summary

Turkey gizzard caldesmon

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Muscle Physiology

Background:

  • Caldesmon is a protein found in smooth muscle.
  • Previous studies reported different molecular weights for chicken and turkey gizzard caldesmon.

Purpose of the Study:

  • To accurately determine the molecular weight of turkey gizzard caldesmon.
  • To compare turkey gizzard caldesmon with other muscle caldesmons.

Main Methods:

  • Sedimentation equilibrium analysis in analytical ultracentrifuge.
  • Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE).

Main Results:

  • The molecular weight of turkey gizzard caldesmon was determined to be 90 +/- 3 kDa.
  • This value is consistent with typical muscle caldesmons, unlike a previously reported lower value.
  • Chicken and turkey muscle caldesmons showed different migration patterns in SDS-PAGE, suggesting distinct amino acid sequences.

Conclusions:

  • Turkey gizzard caldesmon is a typical muscle caldesmon.
  • Turkey gizzard caldesmon has a different amino acid sequence compared to chicken gizzard caldesmon.

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