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Polyunsaturated fatty acids reduce pyrogen-induced tissue factor expression in human monocytes
1Sanofi Recherche, Toulouse, France.
Biochemical Pharmacology
|July 19, 1994
Summary
Polyunsaturated fatty acids inhibit tissue factor expression in monocyte/macrophages, potentially explaining their antithrombotic effects. This inhibition was not observed in human umbilical vein endothelial cells.
Area of Science:
- Biochemistry
- Immunology
- Cardiovascular Research
Background:
- Tissue factor initiates blood coagulation and is implicated in thrombosis and atherosclerosis.
- Endotoxin (LPS) and interleukin-1 beta (IL-1 beta) are known to upregulate tissue factor expression.
Purpose of the Study:
- To investigate the effect of fatty acids on tissue factor expression.
- To explore the role of monocyte/macrophages and endothelial cells in this process.
Main Methods:
- Cultured human umbilical vein endothelial cells (HUVEC) and human monocyte/macrophages were treated with LPS or IL-1 beta.
- The expression of tissue factor was measured following treatment with various fatty acids (oleic, linoleic, linolenic, palmitic acid).
Main Results:
- Oleic acid, linoleic acid, and linolenic acid inhibited LPS-induced tissue factor expression in monocyte/macrophages.
- These fatty acids also reduced the pyrogenic effects of IL-1 beta on monocyte/macrophages.
- No significant effect of these fatty acids on LPS- or IL-1 beta-induced tissue factor expression was observed in HUVEC.
Conclusions:
- Polyunsaturated fatty acids may exert antithrombotic and antiatherogenic effects by inhibiting tissue factor expression in monocyte/macrophages.
- The inhibitory effect appears specific to monocyte/macrophages and not endothelial cells.