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Comparison of hemp and cotton fiber implants in muscle rat tissue. Study of the inflammatory response
S Dorfman1, D Dorfman, R Leonardi
1Servicio de Cirugía General, Hospital General del Sur, Venezuela.
Abstract:
Hemp fiber is obtained from the plant Musa textilis. The cost of preparation of its raw fibers is low. The purpose of this paper was to compare the inflammatory response in the rat muscle tissue originated by both hemp and cotton fibers. Both types of fibers, were implanted in gluteal muscles of Sprague Dawley rats. The rats were sacrificed at 15, 30 and 60 postoperative days. Muscle tissue sections were stained with hematoxilyneosin. The inflammatory response was measured by subtracting the suture surface area from the total granulomatous area. At 15 days, the inflammatory response was more conspicuous for hemp than for cotton fiber (P < 0.05). At 30 and 60 days, responses were similar (P > 0.05). We cannot conclude that the hemp fiber is superior to cotton, nevertheless, they behave the same. Therefore, hemp constitutes an alternative as suture material.
Insights
Hemp and cotton fibers show similar inflammatory responses in rat muscle tissue after implantation. This suggests hemp fiber is a viable alternative to cotton for suture materials.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Surgical Sutures
Background:
- Hemp fiber, derived from Musa textilis, offers a low-cost raw material.
- Cotton fiber is a commonly used material in surgical applications.
- Evaluating the biocompatibility of novel fiber materials is crucial for medical use.
Purpose of the Study:
- To compare the inflammatory response induced by hemp fiber versus cotton fiber in rat muscle tissue.
- To assess the potential of hemp fiber as a biocompatible alternative for suture materials.
Main Methods:
- Hemp and cotton fibers were implanted into the gluteal muscles of Sprague Dawley rats.
- Rats were euthanized at 15, 30, and 60 days post-implantation.
- Inflammatory response was quantified by measuring the granulomatous area and subtracting suture surface area in hematoxylin-eosin stained muscle sections.
Main Results:
- At 15 days postoperative, hemp fiber elicited a significantly greater inflammatory response compared to cotton fiber (P < 0.05).
- By 30 and 60 days postoperative, the inflammatory responses to both hemp and cotton fibers were statistically similar (P > 0.05).
Conclusions:
- Hemp fiber demonstrates comparable tissue response to cotton fiber over time.
- Hemp fiber presents a potential alternative biomaterial for suture applications due to its similar biocompatibility profile.