[Effect of polyhemoglobin composition on erythrocyte aggregation and circulation time in the vascular bed]

Aggregation of erythrocytes and circulation time of polyhemoglobins in blood plasma was estimated in the model of 50% blood loss compensation in vivo and in vitro using solutions of polyhemoglobins with different content of polymerized form (from 2-3% to 70-73%). Hemoglobin derivatives were characterized by HPLC, sedimentation analysis; the main functional properties of polyhemoglobins were evaluated as well as their influence on erythrocytes aggregation in vitro and in vivo on dogs. The optimal content of polymerized form (25-30%) was estimated which provides sufficient prolongation of circulation time and does not enhance the erythrocytes aggregation. The conclusion was made that influence on erythrocyte aggregation likewise functional properties and circulation time may be considered as criterion of optimal fractional composition and biocompatibility of the polyhemoglobins.