Related Experiment Videos
The fractionation of human plasma proteins. I. Affinity purification of human complement properdin
1Department of Immunology, Scripps Research Institute, La Jolla, California 92037.
Protein Expression and Purification
|April 1, 1994
Abstract:
A method was developed for the affinity purification of human complement properdin. The preparation is part of an integrated scheme in which over 20 human plasma proteins can be recovered in a highly purified form. The yield of properdin was 5.9 mg from 3 liters of plasma, amounting to a 28% recovery. The crucial step in the purification was the application of affinity chromatography using C3b-ester-Sepharose.