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Purification of lipases, phospholipases and kinases by heparin-Sepharose chromatography
A A Farooqui1, H C Yang, L A Horrocks
1Department of Medical Biochemistry, Ohio State University, Columbus 43210.
Journal of Chromatography. A
|July 8, 1994
Abstract:
Heparin interacts with lipases, phospholipases and kinases. Immobilized heparin can be used for the purification of diacylglycerol and triacylglycerol lipases, phospholipases A2 and C and protein and lipid kinases. The use of heparin-Sepharose is an important development in analytical and preparative techniques for the separation and isolation of lipases, phospholipases and kinases.