Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Sperm function and its manipulation for microassisted fertilization

S Fleming¹, S Green, J Hall

¹Dept. of Obstetrics and Gynaecology, University Hospital, Queens Medical Centre, Nottingham.

Bailliere'S Clinical Obstetrics and Gynaecology

|March 1, 1994

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Creativity in clinical practice.

Australian nursing journal (July 1993)·2002

Same author

Inhibition of CD40-mediated endothelial cell activation with antisense oligonucleotides.

Transplantation·2002

Same author

Increased expression of unmethylated CDKN2D by 5-aza-2'-deoxycytidine in human lung cancer cells.

Oncogene·2001

Same author

Consequences of the inhibition of Hdm2 expression in human osteosarcoma cells using antisense oligonucleotides.

Anti-cancer drug design·2001

Same author

Health services research in Australia.

Australian health review : a publication of the Australian Hospital Association·2001

Same author

Environmental causes of human cancers.

European journal of cancer (Oxford, England : 1990)·2001

Same journal

The use of gonadotrophin-releasing hormone antagonists in polycystic ovarian disease.

Bailliere's clinical obstetrics and gynaecology·2000

Same journal

Use of luteinizing hormone releasing hormone agonists in polycystic ovary syndrome.

Bailliere's clinical obstetrics and gynaecology·2000

Same journal

Epithelial ovarian cancer, infertility and induction of ovulation: possible pathogenesis and updated concepts.

Bailliere's clinical obstetrics and gynaecology·2000

Same journal

Complications of ovarian stimulation.

Bailliere's clinical obstetrics and gynaecology·2000

Same journal

Adverse effects of luteinizing hormone on fertility: fact or fantasy.

Bailliere's clinical obstetrics and gynaecology·2000

Same journal

Surgical management of polycystic ovarian syndrome.

Bailliere's clinical obstetrics and gynaecology·2000

See all related articles

Understanding sperm function is key for assisted conception, especially microassisted fertilization. New methods to assess sperm fertilization potential and induce acrosome reactions are needed to improve success rates.

Area of Science:

Reproductive biology
Spermatozoa function
Assisted reproductive technology

Background:

Assisted conception and microassisted fertilization bypass natural fertilization events.
Oligoasthenozoospermia management can be aided by methylxanthine derivatives like pentoxifylline.
Spermatozoa motility and acrosome reaction are critical for fertilization.

Purpose of the Study:

To explore novel techniques for improving microassisted fertilization success rates.
To investigate the role of reactive oxygen species and acrosin in sperm function.
To address the inadequacy of current methods for assessing sperm fertilization potential.

Main Methods:

Utilizing methylxanthine derivatives (e.g., pentoxifylline) for oligoasthenozoospermia.

Related Experiment Videos

Generating low concentrations of reactive oxygen species in vitro.

Investigating acrosin activity and acrosome-reacted spermatozoa selection.

Main Results:

Pentoxifylline aids motility in procedures like SUZI.
Reactive oxygen species may be involved in capacitation and hyperactivation.
Acrosin activity monitoring and selection of reacted spermatozoa show promise.

Conclusions:

Improved understanding of sperm function is crucial for assisted conception.
New methods for sperm selection and acrosome reaction induction are needed.
Accurate assessment of fertilization potential will enhance success rates and reduce risks.