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Updated: Aug 16, 2026

Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
Published on: February 14, 2018
Amino- and aminomethylcholesterol derivatives with fungicidal activity
L Elkihel1, I Soustre, F Karst
1Laboratoire de Physicochimie des Substances Naturelles, Université de La Rochelle, France.
Abstract:
Among a series of aminocholesterol derivatives synthesized, 7-aminocholesterol is the strongest inhibitor of yeast cell growth. Using sterol auxotrophic mutant strains, we showed that this compound inhibits cell proliferation by interfering with ergosterol biosynthesis. The sterol pattern of treated cells revealed that 7-aminocholesterol inhibits delta 8-->delta 7-sterol isomerase and delta 14-sterol reductase as morpholine inhibitors. However, the novel feature of this compound is a strong cytotoxicity to yeast.
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