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TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
Published on: October 8, 2012
Isolation of a Vibrio cholerae transposon-mutant with an altered viable but nonculturable response
J Ravel1, R T Hill, R R Colwell
1Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore 21202.
FEMS Microbiology Letters
|July 1, 1994
Abstract:
We have isolated more than 2500 mutants of Vibrio cholerae by using transposon mutagenesis. Mutants were screened under low nutrient conditions in artificial seawater for an altered viable but nonculturable response, compared to the wild-type. Mutant JR09H1 entered the viable but nonculturable state more rapidly than the wild-type at both 25 degrees C and 4 degrees C.
