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Cloning and sequence analysis of the human SNAP25 cDNA
N Zhao1, H Hashida, N Takahashi
1Human Genome Center, University of Tokyo, Japan.
Gene
|August 5, 1994
Summary
Researchers identified a human gene, human synaptosomal-associated protein 25 (hSNAP25), highly expressed in the brain. This gene is a close match to the mouse version, indicating conserved function in brain development and synaptic function.
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- Synaptosomal-associated protein 25 (SNAP25) is crucial for synaptic vesicle exocytosis.
- Previous studies identified mouse SNAP25 (mSNAP25), but its human counterpart was not fully characterized.
Purpose of the Study:
- To identify and characterize human brain-specific genes.
- To isolate and analyze a cDNA clone highly expressed in the human brain.
Main Methods:
- Modified differential hybridization analysis for screening human brain-specific cDNAs.
- Nucleotide sequence analysis of the identified clone.
Main Results:
- A clone highly and specifically expressed in the adult human brain was identified.
- The clone was confirmed to be a nearly full-length cDNA of human synaptosomal-associated protein 25 (hSNAP25).
- hSNAP25 exhibited perfect amino-acid sequence conservation with mSNAP25.
Conclusions:
- hSNAP25 is a human homologue of mSNAP25, highly conserved at the amino acid level.
- The discovery of hSNAP25 provides a valuable tool for studying synaptic function and neurological disorders in humans.