Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA organization in human spermatozoa

J G Barone1, J De Lara, K B Cummings

  • 1Division of Urology, Robert Wood Johnson University Medical School, New Brunswick, New Jersey 08903-0019.

Journal of Andrology
|March 1, 1994
PubMed
Summary

Human sperm DNA is organized into loop domains attached to a nuclear matrix, similar to hamsters. This DNA remains anchored at the tail base, indicating a nuclear annulus-like structure, but it

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Double-stranded DNA breaks hidden in the neutral Comet assay suggest a role of the sperm nuclear matrix in DNA integrity maintenance.

Molecular human reproduction·2013
Same author

Langevin simulation of rf collisional multipactor breakdown of gases.

Physical review. E, Statistical, nonlinear, and soft matter physics·2009
Same author

Sperm DNA fragmentation: awakening the sleeping genome.

Biochemical Society transactions·2007
Same author

In vitro trial of the pilot prototype of the prostate mechanical imaging system.

Urology·2001
Same author

Yeast as a model system for studying endocytosis.

Experimental cell research·2001
Same author

Mitoxantrone in patients with prostate specific antigen progression after local therapy for prostate carcinoma.

Cancer·2001

Area of Science:

  • Reproductive Biology
  • Molecular Biology
  • Genetics

Background:

  • Rodent sperm DNA is packaged into loop domains attached to a nuclear matrix.
  • Upon decondensation, sperm DNA anchors to a nuclear annulus at the tail base.

Purpose of the Study:

  • To investigate human sperm DNA organization and compare it to rodent sperm.
  • To characterize the structure and stability of the human sperm nuclear matrix and annulus.

Main Methods:

  • In situ hybridization using human centromere repeats as probes.
  • Extraction and decondensation of human sperm nuclei.
  • Microscopy to analyze DNA loop domains and nuclear structures.

Main Results:

  • Human sperm DNA is organized into loop domains attached to a nuclear matrix.
  • Human sperm DNA loop domains are smaller than somatic cell domains (approx. 26.8 +/- 2.1 kb).
  • A nuclear annulus-like structure anchors decondensed human sperm DNA, but it is structurally unstable.

Conclusions:

  • Human sperm DNA packaging is highly organized by nuclear structures, similar to hamsters.
  • The human sperm nuclear annulus exists but is less stable than its hamster counterpart.
  • These findings contribute to understanding sperm chromatin organization and its implications.

Related Experiment Videos