Three different cDNAs encoding mouse D-factor/LIF receptor

Area of Science:

• Molecular Biology
• Genetics
• Biochemistry

Background:

• Differentiation-stimulating factor (D-factor), also known as leukemia inhibitory factor (LIF), plays a crucial role in cellular differentiation.
• Understanding the receptor for D-factor/LIF is essential for elucidating its biological functions.

Purpose of the Study:

• To isolate and characterize cDNAs encoding the mouse differentiation-stimulating factor (D-factor)/leukemia inhibitory factor (LIF) receptor.
• To identify potential isoforms of the D-factor/LIF receptor, including soluble forms.

Main Methods:

• Isolation of mouse liver cDNAs using a probe derived from human sequences.
• RT-PCR (Reverse Transcription Polymerase Chain Reaction) for probe preparation.
• Sequence analysis to identify receptor domains and alternative splicing events.

Main Results:

• Three cDNAs for the mouse D-factor/LIF receptor were isolated, showing high homology to the human counterpart.
• A soluble receptor isoform was identified, resulting from a 501 bp insertion that introduces a premature stop codon.
• Alternative polyadenylation of the soluble receptor mRNA leads to two distinct transcript sizes (2.6-3 kb and >5 kb), with shorter transcripts being highly abundant in the liver.

Conclusions:

• The mouse D-factor/LIF receptor exists in at least two forms: a full-length cellular receptor and a soluble receptor.
• The soluble D-factor/LIF receptor is generated through alternative splicing and exhibits differential mRNA processing, with abundant expression in the liver.
• These findings provide insights into the molecular mechanisms regulating D-factor/LIF signaling and receptor availability.